Cloning and analysis of the NBS-LRR gene family in finger millet (Eleusine coracana L.)

Saha, D. and Rana, R.S. (2016) Cloning and analysis of the NBS-LRR gene family in finger millet (Eleusine coracana L.). Plant Knowledge Journal, 5 (1). pp. 1-8.

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Abstract

Nucleotide binding site leucine rich repeat protein (NBS-LRR) is a multi-member gene family in plants encoding important disease resistance proteins to fend against various pathogen infections. Recent advances in genetics and genomics facilitated discovery and functional analysis of NBS-LRR genes or resistance gene homologs (RGHs) in various plant species. In the present work, we have cloned 57 non-identical NBS-LRR sequences from a blast disease resistant finger millet genotype IE1012. We assembled the 57 NBS-LRR sequences to the existing finger millet NBS-LRRs from NCBI Genbank through CAP3 program to obtain a total of 28 sequences. The hidden Markov models (HMMs) analysis further identified 16 NBS-LRRs as EcRGHs with uninterrupted open reading frames (ORFs), of which nine EcRGHs was added from the present work. The secondary structure analysis of protein sequences revealed characteristic conserved motifs, P-loop, Kinase2 and GLPL and other motifs with significant variations. The phylogenetic classification clustered the 16 EcRGH proteins, mostly in the non-TIR group. BLAST-P analysis showed homology of the 16 EcRGHs to NBS-LRR proteins reported across other grass species. The EcRGHs analysed here providing a useful genomic resource for genetic studies of resistance genes in finger millet and exploit them in resistance breeding programs.

Item Type: Article
Uncontrolled Keywords: Finger millet; gene family; NBS-LRR; resistance gene homologs
Author Affiliation: Division of Genomic Resources, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012 INDIA
Subjects: Crop Improvement
Divisions: Millet
Depositing User: Mr T L Gautham
Date Deposited: 12 Jan 2016 04:05
Last Modified: 12 Jan 2016 04:05
URI: http://eprints.icrisat.ac.in/id/eprint/14159

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