Roy, S.K. and Kamal, A.H.M. and Hye-Rim, K. and SooJeong, K. and etl, . (2014) Proteome profiling of seed from inbred and mutant line of sorghum (Sorghum bicolor). Australian Journal of Crop Science, 8 (4). pp. 606-611.
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Abstract
Grain sorghum is a major staple food, with fifth rank among the cereals world-wide, considering its importance for food and feed applications. Cereals are main part of human nutrition and strategic resources. In this study, we executed a comprehensive proteomic study to investigate the seed storage proteins using mature seeds from inbred line (BTx623) and mutant line (M2P1207) of sorghum. Proteins were separated from the mature seeds using IEF in the first-dimension and 2D-PAGE in the second dimension along with MALDI-TOF-TOF mass spectrometry. Two dimensional gels stained with coomassie brilliant blue(CBB), we confirmed 62 differential expressed proteins (≤2-fold) out of 293 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 34 differential expressed protein spots (≥2-fold) were analyzed by mass spectrometry. Out of 34 protein spots, we identified 19 protein spots as up-regulated whereas 15 protein spots as down-regulated. The identified seed storage proteins can economically be used to assess genetic variation and relation in sorghum germplasm
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Seed storage protein; Sorghum bicolor; inbred, and mutant line; proteomics |
| Author Affiliation: | Deptartment of Crop Science, Chungbuk National University, Cheongju 361-763, Korea Republic |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 07 Jan 2016 05:30 |
| Last Modified: | 07 Jan 2016 05:30 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14148 |
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