Barari, L. and Mosavi, N. and Asgharpour, F. and et al, . (2015) Antibacterial and antifungal effect of chickpea (cicer arietinum) aqueous seed extract. World Journal of Pharmacy and Pharmaceutical Sciences, 4 (10). pp. 336-346.
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Abstract
In the present study, 2D electrophoresis, antibacterial and antifungal effects of partially ammonium sulphat precipitation of chickpea (Cicer arietinum) seed proteins were surveyed. The antibacterial activities were evaluated by determining the zone inhibitor, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). The 2D electrophoresis showed iso-electric points of chickpea proteins from pH 5 to 7.5, which was corresponding to neutral and was slightly acidic. Also, the results showed that antibacterial activities towards Gram-positive strains (S. aureus, B. subtilis) were more active than Gram-negative strains (E.coli, P. aeruginosa and K. pneumoniae). Furthermore, B. subtilis was found to be the most sensitive strain based on MBC value. The antifungal effects on R. Solani were estimated by inhibition mycelial growth test. Inhibition mycelial growth test indicated that only 5% ammonium sulphat precipitation proteins inhibited mycelial growth with an IC50 of 60 μg ml-1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chickpea, Anti-proliferative, Plant extract protein, Cell line, Anticancer |
| Author Affiliation: | Department of Biology, University of Mohaghegh Ardabili, P.O.Box 179, Ardabil, Iran. |
| Subjects: | Crop Improvement > Seed Technology |
| Divisions: | Chickpea |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 04 Dec 2015 08:31 |
| Last Modified: | 04 Dec 2015 08:31 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14065 |
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