Rani, K. and Arora, A. (2015) Phenotypic screening and molecular characterization of chickpea genotypes against four different races of fusarium wilt. International Journal of Agricultural Science and Research, 5 (5). pp. 445-454.
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Abstract
The present investigation was undertaken for phenotypic evaluation of six genotypes of chickpea viz. PG043, HC1, DCP92-3, GNG1581, PKG024 and PG071 for resistance to four Foc races (1, 2, 4 and 6) of chickpea wilt pathogen and their molecular characterization. Eight markers viz. four microsatellite based markers (TA110, TA59, TA96 and TA27) linked to resistance allele, 2 RAPD markers: UBC-170 and A07C417, 1 SCAR marker: CS27A700 and 1 ISSR marker: UBC-825 linked to susceptibility, that have been already validated in diverse genetic backgrounds were taken for molecular screening. Amplified allele size for resistance and susceptibility obtained by each primer-genotype combination, were recorded. Genotypes PG043, GNG1581 and PG071 were found to be resistant for three races 1, 2 and 4, while PKG024 was found to be susceptible after phenotypic screening and molecular characterization with all the linked markers. The use of the sorted wilt resistant genotypes can make available ample prospect in MAS breeding of chickpea.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Artificial Screening, Chickpea, Fusarium wilt, Molecular Markers, Resistance |
| Author Affiliation: | Division of Genetics, Indian Agricultural Research Institute (IARI), New Delhi, India |
| Subjects: | Crop Improvement |
| Divisions: | Chickpea |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 04 Dec 2015 08:23 |
| Last Modified: | 04 Dec 2015 08:23 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14052 |
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