Li, J. and Wang, L. and Zhan, Q. and Liu, Y (2015) Map-based cloning and expression analysis of BMR-6 in sorghum. Journal of Genetics, 94 (3). pp. 445-452.
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Abstract
Brown midrib mutants in sorghum are associated with reduced lignin content and increased cell wall digestibility. In this study, we characterized a bmr-6 sorghum mutant, which shows reddish pigment in the midrib and stem after the fifth-leaf stage. Compared to wild type, Kalson lignin content of bmr-6 is decreased significantly. We used histological analysis to determine that the mutant exhibited a modified pattern of lignin staining and found an increased polysaccharide content. We cloned BMR-6 gene, a gene encoded a cinnamyl alcohol dehydrogenase (CAD), using a map-based cloning approach. Genetic complementation confirmed that CAD is responsible for the BMR-6 phenotype. BMR-6 gene was expressed in all tested sorghum tissues, with the highest being in midrib and stem. Transient expression assays in Nicotiana benthamiana leaves demonstrated cytomplasmic localization of BMR-6. We found that the expression level of bmr-6 was significantly decreased in the mutant but expression of SbCAD3 and SbCAD5 were significantly increased. Our results indicate that BMR-6 not only affects the distribution of lignin but also the biosynthesis of lignin in sorghum
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | bmr-6; cinnamyl alcohol dehydrogenase; lignin biosynthesis; sorghum |
| Author Affiliation: | College of Agriculture, Anhui Science and Technology University, Fengyang 233100, People’s Republic of China |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 15 Oct 2015 10:13 |
| Last Modified: | 15 Oct 2015 10:13 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13949 |
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