Sjöblom, M. and Matsakas, L. and Christakopoulos, P. (2015) Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses. Industrial Crops and Products, 74. pp. 535-544.
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Abstract
Enzymatically liquefied sweet sorghum stalks and beet molasses were evaluated for butyrate production using Clostridium tyrobutyricum in 1 L scale fed-batch fermentations. The hydrolysates used for thefermentations were prepared separately by liquefying the sorghum stalks at 50◦C, pH 5.0 for 18 h, with30% (w/v) DM content using the enzyme preparation Cellic®CTec2 at an activity of 26.5 FPU/g DM. Toenhance sucrose consumption, the fermentations were supplemented with invertase at an activity equiv-alent to 8.3 U/g DM. With the hydrolysate as the feedstock, a butyrate concentration of 37.2 ± 0.8 g/L, aproductivity of 0.86 ± 0.02 g/L h and a yield of 0.39 ± 0.02 g/g (p = 0.05) consumed sugars were obtained.Finally, a butyrate concentration of 58.8 g/L, a productivity of 1.9 g/L h, a butyrate yield of 0.52 g/g con-sumed sugars and a dry cell mass concentration of 15.7 g/L were obtained with fed-batch cultivation anda constant feed consisting of 64% sorghum hydrolysate juice and 36% molasses. Evidence for induciblesaccharolytic activity was also proven, as the cellulase activity in the culture supernatant was found morethan double during feed with limiting sugar concentrations. The present study clearly demonstrates that combinations of low cost raw materials can be used for efficient butyrate production, also without cell immobilization.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Butyric acid, Enzymatic liquefaction, Molasses, Saccharolytic activity, Sweet sorghum |
| Author Affiliation: | Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Lulea University of Technology, SE-971 87 Lulea, Sweden |
| Subjects: | Plant Production Plant Protection |
| Divisions: | Sorghum |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 30 Sep 2015 10:00 |
| Last Modified: | 30 Sep 2015 10:00 |
| Official URL: | http://dx.doi.org/10.1016/j.indcrop.2015.05.041 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13913 |
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