Characterization of Erwinia chrysanthemi isolates inciting stalk rot disease of sorghum

Kharayat, B.S. and Singh, Y. (2015) Characterization of Erwinia chrysanthemi isolates inciting stalk rot disease of sorghum. African Journal of Agricultural Research, 10 (22). pp. 2309-2314.

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Abstract

Stalk rot of sorghum plants caused by Erwinia chrysanthemi is one of the most destructive diseases of sorghum crop. Twenty one of bacterial isolates were isolated from stalk of sorghum plants from different location of U.S. Nagar district of Uttarakhand, India. Biochemical, physiological and morphological characterization of E. chrysanthemi was done for confirmation of the bacterium specie. Bacterial suspension [0.7% Tween-40 (v/v) + 2 × 108 cell/ml] of each isolate was inject-inoculated with a 21G hypodermic needle into the stalk of plants for pathogenicity testing of the test bacterium. Reactions of biochemical and physiological testing are clearly evident enough to support the confirmation of test bacterium to taxonomic assignation of E. chrysanthemi causing soft roton sorghum plants. Out of 31 diseased samples of different locations examined, in 21 samples, the pathogen was detected as E. chrysanthemi by using set of biochemical and physiological testing. As all the bacterium produced typical stalk rot disease symptoms on sorghum plant and water-insoluble blue pigment (indigoidine) on nutrient glycerol MnCl2.4H2O (2 mM) agar medium it was confirmed as chrysanthemi species.

Item Type: Article
Uncontrolled Keywords: Blue pigment, Characterization, Erwinia chrysanthemi, Stalk rot, Sorghum
Author Affiliation: Centre of Advance Faculty Training in Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, PIN-263145, U.S. Nagar, Uttarakhand, India.
Subjects: Plant Protection
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 10 Sep 2015 10:23
Last Modified: 10 Sep 2015 10:23
Official URL: http://dx.doi.org/10.5897/AJAR2014.9031
URI: http://eprints.icrisat.ac.in/id/eprint/13870

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