Transmission of sorghum stunt mosaic rhabdovirus by the leafhopper vector, Graminella sonora (Homoptera: Cicadellidae)

Creamer, R. and He, X. (1997) Transmission of sorghum stunt mosaic rhabdovirus by the leafhopper vector, Graminella sonora (Homoptera: Cicadellidae). Plant Disease, 81 (1). pp. 63-65.

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Abstract

The transmission parameters of sorghum stunt mosaic rhabdovirus (SSMV) by the leafhopper Graminella sonora were determined. The minimum acquisition and inoculation times were 6 and 1 h, respectively, while 100% transmission was obtained from a 48-h acquisition access or a 24-h inoculation access period. Transmission efficiency was highest at temperatures of 24 to 36°C. The minimum latent period prior to transmission was 9 days at 30°C, with an 11-day incubation period necessary for 100% transmission. After a 14-day incubation period at 16°C, no transmission was obtained; however, doubling the incubation period to 28 days gave high levels of transmission. Double antibody sandwich enzyme-linked immunosorbent assay (DASELISA) of individual leafhoppers held at 16°C for a 14-day incubation period detected no virus in leafhoppers, whereas over 50% of the leafhoppers had detectable virus after a 28-day incubation period. Graminella nigrifrons and Peregrinus maidis were not able to transmit SSMV

Item Type: Article
Author Affiliation: Department of Plant Pathology, University of California, Riverside 92521; and W. E.
Subjects: Plant Protection > Control Methods
Divisions: Sorghum
Depositing User: David T
Date Deposited: 19 Jan 2011 19:53
Last Modified: 19 Jan 2011 19:53
Official URL: http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS...
URI: http://eprints.icrisat.ac.in/id/eprint/1386

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