Dinakaran, E. and Bharathi Raja, R. and Thiruvengadam, V. and Bapu, J.R.K. and et al, . (2015) Determination of oligosaccharide fraction in a worldwide germplasm collection of chickpea (Cicer arietinum L.) using high performance liquid chromatography. Australian Journal of Crop Science, 9 (7). pp. 605-613.
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Abstract
Awareness on nutritive value and health benefits of legumes is of vital importance in order to increase the consumption of legumes in daily diet of the human beings. Despite of high nutritional value, it contains one of the most important antinutritional compounds such as the Raffinose Family Oligosaccharides (RFOs). The RFOs are predominantly found in the seeds of leguminous crops and are indigestible by humans and animals causing flatulence or diarrhea. In the present study, a total of 213 chickpea accessions were analysed for seed oligosaccharides (Sucrose, Raffinose, Ciceritol, and Stachyose) through high performance liquid chromatography with the aim of selecting promising accessions with low RFOs and high sucrose. Data with three replications was used to measure Pearson’s simple correlation co-efficient in order to establish the relationship among various oligosaccharide fractions. The results showed that the sucrose content ranged from 3.57 mg g-1 to 54.12 mg g-1 with 17 accessions contained more than 35 mg g-1 and 10 accessions had less than 10 mg g-1. The stachyose content ranged from 2.77 mg g-1 to 59.43 mg g-1 with 12 accessions had less than 11 mg g-1. Ciceritol was the main sugar found in all the samples ranged from 4.36 mg g-1 to 90.65 mg g-1. The identified germplasm accessions with unique sugar profiles especially those with low RFO will be valuable in breeding specialty chickpea for improved sugar content
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Germplasm collection, Chick pea, Raffinose family oligosaccharides, Flatulence, Core collection |
| Author Affiliation: | Department of Pulses, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India |
| Subjects: | Crop Improvement |
| Divisions: | Chickpea |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 27 Aug 2015 08:56 |
| Last Modified: | 27 Aug 2015 08:56 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13768 |
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