Mushtaq, S. and Akram, A. and Hanif, N.Q. and Qureshi, R. and et al, . (2015) Natural incidence of aflatoxins, mycological profile and molecular characterization of aflatoxigenic strains in chickpea flour. Pakistan Journal of Botany, 47 (3). pp. 1153-1160.
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Abstract
The mycological profile of retail chickpea flour (locall called Baisan), sold in the markets in the Rawalpindi district was studied. All the samples were tested for the contamination with aflatoxins. A total of 13 fungal species isolated from the flour and out of which, Aspergillus flavus was recorded the most common species (100%), followed by Rhizopus oryzea (50%), Aspergillus niger (40%), Penicilium digitatum (30%), Cladosporium cladosporoides, Fusarium oxysporium, Mucor recemosus, M. petrinsularis and Rhizopus arrhizus (20% each), Aspergillus oryzea, Botritus cinerea, Mucor circineloides and Penicillium sp. (10% each). Aflatoxin B1 was found in only 20% of the samples ranging from 3.03-4.24ppb. The molecular characterization was carried out by using PCR using simple sequence repeats (SSR) primers. The SSR amplification pattern clearly showed the genetic variability among the 10 strains of A. flavus. A dendrogram was generated through MVSP software program. Genotype AF04 was most diverse among all genotypes. The similarity value was ranged between 0.538 (53.8%)-0.938 (93.8%).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chickpea, Molecular characterization, Aflatoxins, Dendrogram, PCR, SSR |
| Author Affiliation: | Department of Botany, Pir Mehr Ali Shah Arid Agriculture university Rawalpindi, Pakistan |
| Subjects: | Plant Protection |
| Divisions: | Chickpea |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 13 Aug 2015 05:14 |
| Last Modified: | 13 Aug 2015 05:14 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13686 |
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