Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food

Peng, J. and Song, S. and Xu, L. and Ma, W. and Liu, L. and Kuang, H. and Xu, C. (2013) Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food. International Journal of Environmental Research and Public Health, 10. pp. 2897-2905.

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Abstract

We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.

Item Type:	Article
Uncontrolled Keywords:	Peanut allergen; Ara h 1; Monoclonal antibody; Sandwich ELISA
Author Affiliation:	State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China.
Subjects:	Plant Production
Divisions:	Groundnut
Depositing User:	Mr B Krishnamurthy
Date Deposited:	04 Aug 2015 08:49
Last Modified:	04 Aug 2015 08:49
Official URL:	http://dx.doi.org/10.3390/ijerph10072897
URI:	http://eprints.icrisat.ac.in/id/eprint/13659

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