A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

Wessel, D. and Flügge, U.I. (1984) A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Analytical Biochemistry, 138 (1). pp. 141-143.

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Abstract

A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the presence of detergents, lipids, salt, buffers, and β-mercaptoethanol

Item Type: Article
Uncontrolled Keywords: Protein precipitation; TX-100; lipids; β-mercaptoethanol; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Author Affiliation: Lehrstuhl für Biochemie der Pflanze, Untere Karspüle 2, 3400 Göttingen, Federal Republic of Germany
Subjects: Social Sciences
Plant Physiology and Biochemistry > Biochemistry
Divisions: General
Depositing User: Ms Ishrath Durafsha
Date Deposited: 04 Feb 2015 05:15
Last Modified: 04 Feb 2015 05:15
Official URL: http://dx.doi.org/10.1016/0003-2697(84)90782-6
URI: http://eprints.icrisat.ac.in/id/eprint/13583

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