Devi, K.T. and Mayo, M.A. and Reddy, K.L.N. and Delfosse, P. and Reddy, G. and Reddy, S.V. and Reddy, D.V.R. (1999) Production and characterization of monoclonal antibodies for aflatoxin B1. Letters in Applied Microbiology, 29 (2). pp. 284-288.
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Abstract
Hybridomas that secreted antibodies for aflatoxin B1 were selected using two immunization protocols referred to as A and B. Protocol A is a standard immunization method and resulted in the selection of only two clones that produced monoclonal antibodies against aflatoxin B1. In protocol B a unique immunization schedule which resulted in the generation of 10 hybridomas is described. Of the 10, one antibody was highly specific to B1, four antibodies reacted equally strongly with B1, G1 and weakly with B2. Another four reacted strongly with B1 and weakly with B2 and G1. One clone reacted equally strongly with B1, G1 and B2. Interestingly all the 10 antibodies showed little or no cross-reaction with G2.
| Item Type: | Article |
|---|---|
| Author Affiliation: | International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India |
| Subjects: | Plant Production Soil Science and Microbiology > Microbiology |
| Divisions: | Other Crops |
| Depositing User: | Sandhya Gir |
| Date Deposited: | 12 Jan 2011 16:59 |
| Last Modified: | 12 Jan 2011 16:59 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/1358 |
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