CISprimerTOOL: software to implement a comparative genomics strategy for the development of conserved intron scanning (CIS) markers

Jayashree, B. and Jagadeesh, V.T. and Hoisington, D.A. (2008) CISprimerTOOL: software to implement a comparative genomics strategy for the development of conserved intron scanning (CIS) markers. Molecular Ecology Resources, 8 (3). pp. 575-577.

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Abstract

The availability of complete, annotated genomic sequence information in model organisms is a rich resource that can be extended to understudied orphan crops through comparative genomic approaches. We report here a software tool (CISprimerTOOL) for the identification of conserved intron scanning regions using expressed sequence tag alignments to a completely sequenced model crop genome. The method used is based on earlier studies reporting the assessment of conserved intron scanning primers (called CISP) within relatively conserved exons located near exon-intron boundaries from onion, banana, sorghum and pearl millet alignments with rice. The tool is freely available to academic users at www.icrisat.org/gt-bt/ CISPTool.htm.

Item Type: Article
Author Affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India
Subjects: Crop Improvement > Biotechnology
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 12 Jan 2011 16:16
Last Modified: 12 Jan 2011 16:17
URI: http://eprints.icrisat.ac.in/id/eprint/1356

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