Direct delivery of inoculum to shoot tissue interferes with genotypic resistance to Ralstonia solanacearum in tomato seedlings

Thomas, P. and Sadashiva, A.T. and Upreti, R. and Mujawar, M.M. (2014) Direct delivery of inoculum to shoot tissue interferes with genotypic resistance to Ralstonia solanacearum in tomato seedlings. Journal of Phytopathology. pp. 1-4.

[img] PDF (Online First) - Accepted Version
Restricted to ICRISAT researchers only

Abstract

Employing known susceptible and resistant genotypes and pure bacterial inoculum (0.1 OD; 108 CFU/ml−1), five different inoculation methods were tried to assess the response of tomato genotypes to Ralstonia solanacearum. This included seed-soaking inoculation, seed-sowing followed by inoculum drenching, or at 2-week stage through petiole-excision inoculation, soaking of planting medium with inoculum either directly or after imparting seedling root-injury. Seed-based inoculations or mere inoculum drenching at 2 weeks did not induce much disease in seedlings. Petiole inoculation induced 90–100% mortality in susceptible checks but also 50–60% mortality in normally resistant genotypes within 7–10 days. Root-injury inoculation at 2-week seedling stage appeared the best for early and clearer distinction between resistant and susceptible lines. The observations suggest a role played by the root system in governing genotypic resistance to the pathogen. Direct shoot inoculation is to be adopted only for selecting highly resistant lines or to thin down segregating populations during resistance breeding.

Item Type: Article
Uncontrolled Keywords: Bacterial wilt; disease resistance; petiole inoculation; protray screening; Ralstonia solanacearum; resistance break-down; screening methodology; Solanum lycopersicum
Author Affiliation: Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, India
Subjects: Plant Protection
Divisions: Other Crops
Depositing User: Ms K Syamalamba
Date Deposited: 06 Oct 2014 06:17
Last Modified: 06 Oct 2014 06:17
Official URL: http://dx.doi.org/10.1111/jph.12281
URI: http://eprints.icrisat.ac.in/id/eprint/13480

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.