Thomas, P. and Mujawar, M.M. and Sekhar, A.C. and Upreti, R. (2014) Physical impaction injury effects on bacterial cells during spread plating influenced by cell characteristics of the organisms. Journal of Applied Microbiology, 116 (4). pp. 911-922.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
Restricted to ICRISAT researchers only |
Abstract
AIMS: To understand the factors that contribute to the variations in colony-forming units (CFU) in different bacteria during spread plating. METHODS AND RESULTS: Employing a mix culture of vegetative cells of ten organisms varying in cell characteristics (Gram reaction, cell shape and cell size), spread plating to the extent of just drying the agar surface (50-60 s) was tested in comparison with the alternate spotting-and-tilt-spreading (SATS) approach where 100 μl inoculum was distributed by mere tilting of plate after spotting as 20-25 microdrops. The former imparted a significant reduction in CFU by 20% over the spreader-independent SATS approach. Extending the testing to single organisms, Gram-negative proteobacteria with relatively larger cells (Escherichia, Enterobacter, Agrobacterium, Ralstonia, Pantoea, Pseudomonas and Sphingomonas spp.) showed significant CFU reduction with spread plating except for slow-growing Methylobacterium sp., while those with small rods (Xenophilus sp.) and cocci (Acinetobacter sp.) were less affected. Among Gram-positive nonspore formers, Staphylococcus epidermidis showed significant CFU reduction while Staphylococcus haemolyticus and actinobacteria (Microbacterium, Cellulosimicrobium and Brachybacterium spp.) with small rods/cocci were unaffected. Vegetative cells of Bacillus pumilus and B. subtilis were generally unaffected while others with larger rods (B. thuringiensis, Brevibacillus, Lysinibacillus and Paenibacillus spp.) were significantly affected. A simulated plating study coupled with live-dead bacterial staining endorsed the chances of cell disruption with spreader impaction in afflicted organisms...
| Item Type: | Article |
|---|---|
| Author Affiliation: | Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, India. |
| Subjects: | Soil Science and Microbiology > Microbiology |
| Divisions: | General |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 06 Oct 2014 06:07 |
| Last Modified: | 06 Oct 2014 06:07 |
| Official URL: | http://dx.doi.org/10.1111/jam.12412 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13479 |
Actions (login required)
 |
View Item |
