Kumar, P.L. and Duncan, G.H. and Roberts, I.M. and Jones, A.T. and Reddy, D.V.R. (2002) Cytopathology of pigeonpea sterility mosaic virus in pigeonpea and Nicotiana benthamiana: similarities with those of eriophyid mite-borne agents of undefined aetiology. Annals of Applied Biology, 140 (1). pp. 87-96.
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Abstract
Pigeon pea sterility mosaic virus (PPSMV) is transmitted by the eriophyid mite, Aceria cajani, and is very closely associated with sterility mosaic disease (SMD) of pigeon pea (Cajanus cajan) in the Indian subcontinent. Antiserum produced to purified PPSMV preparations detected a virus-specific 32-kDa protein in the sap of SMD-affected pigeon pea plants by ELISA and Western blotting. PPSMV was transmitted mechanically in the sap of SMD-affected pigeon pea leaves to Nicotiana benthamiana. Ultrastructural studies of symptom-bearing leaves of two pigeon pea cultivars, (ICP8863 and ICP2376) and N. benthamiana infected with PPSMV, detected quasi-spherical, ~100-150 nm membrane-bound bodies and amorphous electron-dense material (EDM). These structures were distributed singly or in groups, in the cytoplasm of all cells, except those in conductive tissues. Fibrous inclusions (FIs), composed of randomly dispersed fibrils with electron-lucent areas, were present in the cytoplasm of palisade cells and rarely in mesophyll cells of the two pigeon pea cultivars but were not detected in infected N. benthamiana plants. In the PPSMV-infected pigeon pea cultivars and N. benthamiana, immunogold labelling, using antiserum to PPSMV, specifically labelled the MBBs and associated EDM, but not the FIs. The MBBs and associated inclusions are similar in appearance to those reported for plants infected with the eriophyid mite-transmitted High Plains virus and the agents of unidentified aetiology associated with rose rosette, fig mosaic, thistle mosaic, wheat spot chlorosis and yellow ringspot of budwood. The nature of these different inclusions is discussed
| Item Type: | Article |
|---|---|
| Author Affiliation: | International Crops Research Institute for the Semi-Arid Tropics, Patancheru - 502 324, India |
| Subjects: | Plant Protection > Pests Crop Improvement |
| Divisions: | Pigeonpea |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 11 Jan 2011 22:15 |
| Last Modified: | 11 Jan 2011 22:15 |
| Official URL: | http://dx.doi.org/10.1111/j.1744-7348.2002.tb00160... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/1344 |
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