Singh, D. and Jamal, F. and Pandey, P.K. (2014) KINETIC ASSESSMENT AND EFFECT ON DEVELOPMENTAL PHYSIOLOGY OF A TRYPSIN INHIBITOR FROM Eugenia jambolana (JAMBUL) SEEDS ON Helicoverpa armigera (HÜBNER). Archives of Insect Biochemistry and Physiology, 85 (2). pp. 94-113.
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Abstract
A trypsin inhibitor was purified from the seeds of Eugenia jambolana (Jambul) with a fold purification of 14.28 and a yield recovery of 2.8%. Electrophoretic analysis of E. jambolana trypsin inhibitor (EjTI) revealed a molecular weight of approximately 17.4 kDa on 12% denaturing polyacrylamide gel electrophoresis with or without reduction. EjTI exhibited high stability over a wide range of temperatures (4–80 °C for 30 min) and pH (3.0–10.0) and inhibited trypsin-like activities of the midgut proteinases of fourth instar Helicoverpa armigera larvae by approximately 86%. Feeding assays containing 0.05, 0.15, and 0.45 (% w/w) EjTI on functionally important fourth-instar larvae indicated a dose-dependent downfall in the larval body weight as well as on extent of survival. The nutritional analysis suggests that EjTI exerts toxic effects on H. armigera. Dixon plot analysis revealed competitive inhibition of larval midgut proteinases by EjTI, with an inhibition constant (Ki) of approximately 3.1 × 10−9 M. However, inhibitor kinetics using double reciprocal plots for trypsin inhibition demonstrated a mixed inhibition pattern. These observations suggest the potential of E. jambolana trypsin inhibitor protein in insect pest management
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Eugenia jambolana; H. armigera; Feeding assays; Kinetics; Stability; Nutritional index |
| Author Affiliation: | Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh, India |
| Subjects: | Plant Protection Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | General |
| Depositing User: | Ms Ishrath Durafsha |
| Date Deposited: | 12 Aug 2015 04:55 |
| Last Modified: | 12 Aug 2015 04:55 |
| Official URL: | http://dx.doi.org/10.1002/arch.21147 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13352 |
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