Gahloth, D. and Shukla, U. and Birah, A. and et al, . (2011) Bioinsecticidal activity of Murraya koenigii miraculin-like protein against Helicoverpa armigera and Spodoptera litura. Archives of Insect Biochemistry and Physiology, 78 (3). pp. 132-144.
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Abstract
Miraculin-like proteins, belonging to the Kunitz superfamily, are natural plant defense agents against pests and predators, and therefore are potential biopesticides for incorporation into pest-resistant crops. Here, a miraculin-like protein from Murraya koenigii was assessed for its in vitro and in vivo effects against two polyphagous lepidopteran insect pests, Helicoverpa armigera and Spodoptera litura. M. koenigii miraculin-like protein (MKMLP) inhibited the trypsin-like activity and total protease activity of H. armigera gut proteinases (HGP) by 78.5 and 40%, respectively, and S.litura gut proteinases (SGP) by 81 and 48%, respectively. The inhibitor was stable and actively inhibited the proteolysis of both HGP and SGP enzymes for up to 72 h. Incorporation of MKMLP into artificial diet adversely affected the growth and development of pests in a dose-dependent manner. After 10 days of feeding on diets containing 200 µM MKMLP, larval weight was reduced to 69 and 44.8% and larval mortality was increased to 40 and 43.3% for H. armigera and S litura, respectively. The LC50 of MKMLP was 0.34 and 0.22% of the diet for H.armigera and S. litura, respectively. These results demonstrate the efficacy of MKMLP as a potential plant defense agent against H. armigera and S. litura
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Murraya koenigii; Miraculin-like protein; Proteinase inhibitors; Bioinsecticidal activity; Lepidopteran pests |
| Author Affiliation: | Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttaranchal, India |
| Subjects: | Plant Protection Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | General |
| Depositing User: | Ms Ishrath Durafsha |
| Date Deposited: | 12 Aug 2015 04:57 |
| Last Modified: | 12 Aug 2015 04:57 |
| Official URL: | http://dx.doi.org/10.1002/arch.20448 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13351 |
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