Tek, A.L. and Kashihara, K. and Murata, M. and Nagaki, K. (2010) Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon. Chromosome Research, 18 (3). pp. 337-347.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
- Accepted Version
Restricted to ICRISAT researchers only |
Abstract
The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Soybean, Centromere-specific histone H3, Kinetochore Satellite repeat, Centromere-associated retrotransposon of Glycine max (GmCR) |
| Author Affiliation: | Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan |
| Subjects: | Crop Improvement Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Soyabean |
| Depositing User: | Ms Ishrath Durafsha |
| Date Deposited: | 25 Jun 2014 03:56 |
| Last Modified: | 25 Jun 2014 03:56 |
| Official URL: | http://dx.doi.org/10.1007/s10577-010-9119-x |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13269 |
Actions (login required)
 |
View Item |
