Singh, V.V. and Verma, V. and Aniruddh, K.P. and Mathur, M. and Yadav, R.S. and Kumar, S. (2009) Optimization and development of regeneration and transformation protocol in Indian mustard using lectin gene from chickpea [Cicer arietinum (L.)]. Journal of Plant Breeding and Crop Science, 1 (9). pp. 306-310.
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Abstract
A simple and easy protocol for regeneration and genetic transformation of Brassica juncea (L.) Czern and Coss cv. Pusa bold has been developed. The optimum regeneration was found on combination of BAP (3.0 mg/l) and IAA (0.2 mg/l). The genetic transformation protocol has been standardized using Agrobacterium tumefaciens (gv3101). Cotyledonary petioles used as explant were pre-cultured for 48 h and than co-cultivated with Agrobacterium suspension for 2 days. Putative transformants were selected from selection medium containing 20 mg/l Kanamycine and 250 mg/l cefotaxime. Complete plant formed after placing shoots on rooting medium (0.5 mg/l IBA). Confirmation of integration of transgene was done by PCR using nptII gene primer
| Item Type: | Article |
|---|---|
| Author Affiliation: | Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan 321 303, India. |
| Subjects: | Plant Production > Production Practices |
| Divisions: | Chickpea |
| Depositing User: | David T |
| Date Deposited: | 10 Jan 2011 21:35 |
| Last Modified: | 10 Jan 2011 21:35 |
| Official URL: | http://www.academicjournals.org/jpbcs/PDF/pdf2009/... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/1319 |
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