Marita, J.M. and Hatfield, R.D. and Rancour, D.M. (2014) Identification and suppression of the p-coumaroyl-CoA: hydroxycinnamyl alcohol transferase in Zea mays L. The Plant Journal. pp. 1-47.
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Abstract
Grasses, such as Zea mays L. (maize), contain relatively high levels of p-coumarates (pCA) within their cell walls. Incorporation of pCA into cell walls is believed to be due to a hydroxycinnamyl transferase that couples pCA to monolignols. To understand the role of pCA in maize development, the p-coumaroyl-CoA: hydroxycinnamyl alcohol transferase (pCAT) was isolated and purified from maize stems. Purified pCAT was subjected to partial trypsin digestion and peptides sequenced by tandem mass spectrometry. T-BLASTN analysis of the acquired peptide sequences identified a single full-length maize cDNA clone encoding all peptide sequences obtained from the purified enzyme. The cDNA clone was obtained and used to generate an RNAi construct for suppressing pCAT expression in maize. We report here on the suppression of the p-coumaroyl-CoA: hydroxycinnamyl alcohol transferase in maize. Primary screening of transgenic maize seedling leaves with a new rapid analytical platform was used to identify plants with decreased amounts of pCA. Using this screening method, mature leaves from fully developed plants were analyzed to confirm reduced pCA levels throughout plant development. Complete analysis of isolated cell walls from mature transgenic stems and leaves revealed lignin levels did not change, but pCA levels decreased and the lignin composition was altered. Transgenics with the lowest levels of pCA had decreased levels of syringyl (S) units in the lignin. Altering the levels of pCAT expression in maize can lead to altered lignin composition, but does not appear to alter the total amount of lignin present in the cell walls.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | p-coumarate; transferase; cell wall; lignin; p-coumaroylation; Zea mays L. |
| Author Affiliation: | USDA-Agricultural Research Service, U.S. Dairy Forage Research Center, Madison, WI, United States |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 28 Apr 2014 06:14 |
| Last Modified: | 28 Apr 2014 06:14 |
| Official URL: | http://dx.doi.org/10.1111/tpj.12510 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13101 |
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