Cost-Effective Transfer of Recessive Traits via the Backcross Procedure

Isleib, T.G. (1997) Cost-Effective Transfer of Recessive Traits via the Backcross Procedure. Crop Science, 37. pp. 139-144.

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Abstract

The backcross breeding method is often used to transfer recessive traits controlled by one or a few genes from one pure line to another. Guidelines are needed to help plant breeders to use backcrossing cost-effectively. In a backcrossingp rograma, plant breeder mayc hoose between methods with two or three stages per cycle. The two-stage method utilizes alternate backcrossing and selfing to identify BC,Sj plants with the recessive trait while the three-stage method uses two sequential crosses followed by selfing. In the three-stage method, nmln, the minimum number of plants required to recover at least one or two BC,Sj plants in the ith cycle with a given probability (1 - ct), is obtained by growing only one BC,S~ plant for each of nai~ BCIS0. Alternative values for the number of BC,S0 plants (rib) and BC,Sj plants per BC,So (nJ are present for a = 0.05 and ct = 0.01 for traits controlled by one, two, three, or four recessive genetic loci. A method to compare the cost-effectiveness of alternative values is presented for cases where the relative costs of crossing, selfing, and evaluation of S/progeny are known. Unless time is the paramount concern, it is only in cases where the ressive trait is controlled by a single locus that it can be more cost-effective to make two sequential crosses to the recurrent parent before selflng than to cross and self and then only if the cost of evaluating BC,Sj plants is high relative to the cost of producing BCiS0 plants. When using the three-stage backcross method, the breeder can reduce the nb and increase ns if the cost of evaluating BC~j plants is low relative to the cost of producing BCIS0 plants.

Item Type: Article
Author Affiliation: N.C. State Univ., Raleigh, NC
Subjects: Crop Improvement
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 14 Apr 2014 08:49
Last Modified: 14 Apr 2014 08:49
Official URL: http://dx.doi.org/10.2135/cropsci1997.0011183X0037...
URI: http://eprints.icrisat.ac.in/id/eprint/13062

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