Naimuddin, . and Akram, M. and Duraimurugan, P. (2009) Detection of a tospovirus associated with necrotic leaf curl disease of mungbean at Kanpur. Journal of Food Legumes, 22 (2). pp. 86-90.
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Abstract
The causal virus of a necrotic leaf curl disease of mungbean at Kanpur was mechanically sap transmissible from field infected mungbean to healthy mungbean and cowpea. Reverse transcriptase-PCR using primers for amplification of nucleocapsid protein (NP) gene of Groundnut bud necrosis virus (GBNV), a tospovirus yielded a single band of ~800 bp. Comparison of the virus under study with the isolates of GBNV from leguminous hosts revealed 97% homology with GBNV – Type isolate, GBNV-MB and GBNV-VU at nucleotide level. At amino acid level, the present virus isolate showed 99% homology with GBNV-Type and GBNV-MB and 98% with GBNV-Mb and 96% with GBNV-VU and GBNV-SE. Sequence analysis of amplified product revealed that the virus causing necrotic leaf curl disease of mungbean at Kanpur is a strain of GBNV and has been named as GBNV-MBKN.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GBNV, Mungbean, Tospovirus, Necrotic leaf curl |
| Author Affiliation: | Indian Institute of Pulses Research, Kanpur |
| Subjects: | Plant Protection Soil Science and Microbiology > Microbiology Crop Improvement > Genetics/Genomics |
| Divisions: | Other Crops |
| Depositing User: | Ms Ishrath Durafsha |
| Date Deposited: | 19 May 2014 10:14 |
| Last Modified: | 19 May 2014 10:14 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/13005 |
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