The Dermal Layer of Sweet Sorghum (Sorghum bicolor) Stalk, a Byproduct of Biofuel Production and Source of Unique 3-Deoxyanthocyanidins, has more Anti-proliferative and Pro-apoptotic Activity than Pith in p53 Variants of HCT116 and Colon Cancer Stem Cells

Massey, A.R. and Reddivari, L. and Vanamala, J. (2014) The Dermal Layer of Sweet Sorghum (Sorghum bicolor) Stalk, a Byproduct of Biofuel Production and Source of Unique 3-Deoxyanthocyanidins, has more Anti-proliferative and Pro-apoptotic Activity than Pith in p53 Variants of HCT116 and Colon Cancer Stem Cells. Journal of Agricultural and Food Chemistry. pp. 1-37.

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Abstract

There is a growing interest in the utilization of sweet sorghum as a renewable resource for biofuels. During the biofuel production process, large quantities of biomass are generated creating a rich source of bioactive compounds. However, knowledge of sweet sorghum stalk is lacking. We measured phenolic content (Folin-Ciocalteu assay), anti-oxidant activity (ABTS) and phytochemical composition (LC-MS) in both pith and dermal layer of the stalk. We further tested the anti-proliferative (Brdu) and pro-apoptotic activities (TUNEL) of these extracts using HCT116 and colon cancer stem cells (CCSC) with and without the tumor suppressor gene p53. For the first time, we show that dermal layer extract of sweet sorghum contains more of the 3-deoxyanthocyanidins, apigeninidin and luteolinidin, than pith and this was associated with more anti-cancer activity. Furthermore, luteolinidin suppressed CCSC proliferation more than apigeninidin. In addition to being renewable biofuel, sweet sorghum may also serve as source of health-promoting compounds.

Item Type: Article
Additional Information: This research was funded by Great Valley Energy LLC, Bakersfield, CA.
Uncontrolled Keywords: sweet sorghum, Sorghum bicolor, biorefinery, biofuel, total phenolics, antioxidants, LC-MS, proliferation, apoptosis, HCT116, cancer stem cell, p53, apigeninidin, luteolinidin
Author Affiliation: Colorado State University, USA
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr Siva Shankar
Date Deposited: 25 Mar 2014 09:02
Last Modified: 25 Mar 2014 10:23
Official URL: http://dx.doi.org/10.1021/jf405415u
URI: http://eprints.icrisat.ac.in/id/eprint/12954

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