Clark, L.V. and Jasieniuk, M. (2011) polysat: an R package for polyploid microsatellite analysis. Molecular Ecology Resources, 11 (3). pp. 562-566.
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Abstract
We present an R package to help remedy the lack of software for manipulating and analysing autopolyploid and allopolyploid microsatellite data. polysat can handle genotype data of any ploidy, including populations of mixed ploidy, and assumes that allele copy number is always ambiguous in partial heterozygotes. It can import and export genotype data in eight different formats, calculate pairwise distances between individuals using a stepwise mutation and infinite alleles model, estimate ploidy based on allele counts and estimate allele frequencies and pairwise FST values. This software is freely available through the Comprehensive R Archive Network (http://cran.r-project.org/) and includes a thorough tutorial.
Item Type: | Article |
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Additional Information: | Supporting information: http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0998.2011.02985.x/suppinfo |
Uncontrolled Keywords: | allele copy number ambiguity; data conversion; FST; genetic distance; polyploidy; SSR |
Author Affiliation: | Department of Plant Sciences, Mail Stop 4, University of California, Davis, CA 95616, USA |
Subjects: | Crop Improvement > Genetics/Genomics |
Divisions: | General |
Depositing User: | Mr Siva Shankar |
Date Deposited: | 19 Mar 2014 03:04 |
Last Modified: | 19 Mar 2014 03:04 |
Official URL: | http://dx.doi.org/10.1111/j.1755-0998.2011.02985.x |
URI: | http://eprints.icrisat.ac.in/id/eprint/12908 |
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