Chern, M. and Richter, T. and Ronald, P.C. (2007) Yeast Two-Hybrid Approaches to Dissecting the Plant Defense Response. In: Plant-pathogen interactions : methods and protocols. Methods in molecular biology, . Humana Press Inc., USA, pp. 79-83. ISBN 978-1-58829-448-7
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Abstract
We describe a reliable GAL4-based yeast two-hybrid system for identifying and isolating clones encoding proteins interacting with a protein of interest. This two-hybrid system gives extremely low background and few false-positive clones, making it ideal for library screening purposes. We have successfully used it not only to isolate Arabidopsis NPR1- interactors from rice but also to pull out the rice NPR1 ortholog using one of the interactors as bait.
| Item Type: | Book Section |
|---|---|
| Uncontrolled Keywords: | Yeast two-hybrid; GAL4; NPR1; defense |
| Author Affiliation: | Department of Plant Pathology , University of California at Davis, Davis, CA |
| Subjects: | Crop Improvement |
| Divisions: | General |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 18 Mar 2014 09:23 |
| Last Modified: | 18 Mar 2014 09:23 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/12900 |
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