Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco

Fulnecek, J. and Matyasek, R. and Votruba, I. and et al, . (2011) Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco. Molecular Genetics Genomics, 285. pp. 225-236.

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Abstract

Developmental processes are closely connected to certain states of epigenetic information which, among others, rely on methylation of chromatin. S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are key cofactors of enzymes catalyzing DNA and histone methylation. To study the consequences of altered SAH/ SAM levels on plant development we applied 9-(S)-(2,3- dihydroxypropyl)-adenine (DHPA), an inhibitor of SAHhydrolase, on tobacco seeds during a short phase of germination period (6 days). The transient drug treatment induced: (1) dosage-dependent global DNA hypomethylation mitotically transmitted to adult plants; (2) pleiotropic developmental defects including decreased apical dominance, altered leaf and flower symmetry, flower whorl malformations and reduced fertility; (3) dramatic upregulation of floral organ identity genes NTDEF, NTGLO and NAG1 in leaves. We conclude that temporal SAH-hydrolase inhibition deregulated floral genes expression probably via chromatin methylation changes. The data further show that plants might be particularly sensitive to accurate

Item Type: Article
Uncontrolled Keywords: DNA methylation, Nicotiana tabacum, Plant development � S-adenosyl-L-homocysteine hydrolase inhibitor, DHPA, MADS box genes, DEFICIENS/APETALA 3, GLOBOSA/PISTILLATA, PLENA/ AGAMOUS, FLORICAULA/LEAFY
Author Affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic,
Subjects: Plant Protection
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 14 Mar 2014 09:25
Last Modified: 14 Mar 2014 09:25
Official URL: http://dx.doi.org/10.1007/s00438-011-0601-8
URI: http://eprints.icrisat.ac.in/id/eprint/12876

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