Striking genetic similarity between races of Fusarium oxysporum f. sp. ciceris confirms a monophyletic origin and clonal evolution of the chickpea vascular wilt pathogen

Demers, J.E. and Garzon, C.D. and Jiménez-Gasco, M.M. (2014) Striking genetic similarity between races of Fusarium oxysporum f. sp. ciceris confirms a monophyletic origin and clonal evolution of the chickpea vascular wilt pathogen. European Journal of Plant Pathology. pp. 1-16.

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Abstract

Eight pathogenic races, determined based on the virulence displayed on differential chickpea cultivars, have been recognized in Fusarium oxysporum f. sp. ciceris, the causal agent of Fusarium wilt of chickpea. In order to elucidate the genetic relationships between these races and understand how virulence evolved, we analyzed the sequences of 32 genomic regions for each of the eight races. Twelve of these regions were newly-designed microsatellite markers polymorphic for the F. oxysporum complex (10 displaying polymorphisms in the number of core repeats, and two displaying polymorphic nucleotides in the microsatellite flanking regions), developed from a microsatellite enriched genomic library. The translation elongation factor 1-α (TEF), internal transcribed spacer region of the rDNA (ITS), five mitochondrial regions, a xylanase gene (xyl4) and its transcriptional activator (xlnR), two F. oxysporum f. sp. ciceris sequence characterized amplified regions (SCAR) and 11 microsatellites were completely identical for all races.

Item Type: Article
Additional Information: Pennsylvania State University Graduate Fellowship program and a Microbial Functional Genomics Training Fellowship funded through a USDA grant
Uncontrolled Keywords: Fusarium wilt, Microsatellites,Molecular markers, SSR, Fungal evolution
Author Affiliation: The Pennsylvania State University, University Park, PA 16802, USA
Subjects: Plant Physiology and Biochemistry > Plant Physiology
Plant Physiology and Biochemistry > Biochemistry
Divisions: Chickpea
Depositing User: Mr Siva Shankar
Date Deposited: 14 Mar 2014 05:31
Last Modified: 14 Mar 2014 05:31
Official URL: http://dx.doi.org/10.1007/s10658-014-0387-8
URI: http://eprints.icrisat.ac.in/id/eprint/12866

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