Chamberlin, K.D. and Barkley, N.A. and Tillman, B.L. and et al, .
(2014)
A comparison of methods used to determine the oleic/linoleic acid ratio in cultivated peanut (Arachis hypogaea L.).
Agricultural Sciences, 5 (3).
pp. 227-237.
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Abstract
There is an increasing demand in the peanut
industry for high oleic peanuts and also for the
incorporation of the high oleate trait into newly
released varieties. Early generation screening of
breeding lines for high oleic acid content greatly
increases the efficiency of developing new peanut
varieties. The objective of this study was to
compare the accuracy of methods used to classify
individual peanut seed as high oleic or not
high oleic. Results from capillary electrophoresis
(CE), two variations of near-infrared reflectance
spectroscopy (NIRS), and real-time polymerase
chain reaction (RT-PCR) genotyping,
were compared with the traditionally accepted
reference standard results from gas chromatography
(GC). Three hundred and seventy-four
(374) seeds, spanning twenty-three (23) genotypes
and all four peanut market-types (runner,
Spanish, Valencia and Virginia), were individually
tested by each method. Percent accuracy
levels for rating individual seed as high oleic (H)
ranged from 97.4% (NIRS) to 99.5% (CE). All of
the methods examined in this study carry only a
minor risk for miss-classification (loss of material)
and are suitable for use by peanut breeding
programs in early generation breeding line
screening
| Item Type: |
Article
|
| Additional Information: |
Funding for this project was provided by the USDA-ARS and the University of Florida |
| Uncontrolled Keywords: |
High Oleic Acid; Peanut; Fatty Acid; Gas
Chromatography; Capillary Electrophoresis;
Near Infrared Spectroscopy; Real Time PCR |
| Author Affiliation: |
United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Stillwater, USA |
| Subjects: |
Crop Improvement |
| Divisions: |
Groundnut |
| Depositing User: |
Mr T L Gautham
|
| Date Deposited: |
08 Mar 2014 09:40 |
| Last Modified: |
08 Mar 2014 09:40 |
| Official URL: |
http://dx.doi.org/10.4236/as.2014.53026 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/12844 |
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