Wu, G. and Truksa, M. and Datla, N. and et al, . (2005) Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants. Nature Biotechnology , 23. pp. 1013-1017.
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Abstract
Very long chain polyunsaturated fatty acids (VLCPUFAs) such as arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are valuable commodities that provide important human health benefits1, 2, 3, 4, 5. We report the transgenic production of significant amounts of AA and EPA in Brassica juncea seeds via a stepwise metabolic engineering strategy. Using a series of transformations with increasing numbers of transgenes, we demonstrate the incremental production of VLCPUFAs, achieving AA levels of up to 25% and EPA levels of up to 15% of total seed fatty acids. Both fatty acids were almost exclusively found in triacylglycerols, with AA located preferentially at sn-2 and sn-3 positions and EPA distributed almost equally at all three positions. Moreover, we reconstituted the DHA biosynthetic pathway in plant seeds, demonstrating the practical feasibility of large-scale production of this important ω-3 fatty acid in oilseed crops.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | polyunsaturated fatty acids, Green factories, Oilseed plants, |
| Author Affiliation: | Bioriginal Food & Science Corporation, 110 Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N 0W9. |
| Subjects: | Postharvest Management > Food Technology Crop Improvement Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Other Crops |
| Depositing User: | Ms Ishrath Durafsha |
| Date Deposited: | 23 Feb 2014 10:18 |
| Last Modified: | 23 Feb 2014 10:18 |
| Official URL: | http://dx.doi.org/10.1038/nbt1107 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/12585 |
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