Herald, T.J. and Gadgil, P. and Perumal, R. and et al, . (2014) High-throughput micro-plate HCl–vanillin assay for screening tannin content in sorghum grain†. Journal of The Science of Food and agriculture. pp. 1-4.
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Abstract
The HCl–vanillin assay is a well-accepted method for determining tannin content in sorghum but is limited to small sample sets due to the time-consuming nature of the method. The objective was to develop an accurate and repeatable high-throughput 96-well plate assay for breeders to screen large sample sets of sorghum for tannin content. Validation of the high-throughput assay was tested on 25 sorghums suspected to contain tannin.
| Item Type: | Article |
|---|---|
| Additional Information: | This paper is Contribution No. 14-051-J from the Kansas Agricultural Experiment Station, Manhattan. |
| Uncontrolled Keywords: | tannin,sorghum,96-well plate, vanillin |
| Author Affiliation: | Published 2013. This article is a U.S. Government work and is in the public domain in the USA. |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr Daneti Raju |
| Date Deposited: | 09 Feb 2014 13:34 |
| Last Modified: | 09 Feb 2014 13:34 |
| Official URL: | http://dx.doi.org/10.1002/jsfa.6538 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/12489 |
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