Response of chickpea genotypes to Agrobacterium-mediated delivery of Chickpea chlorotic dwarf virus (CpCDV) genome and identification of resistance source

Kanakala, S. and Verma, H.N. and Vijay, P. and et al, . (2013) Response of chickpea genotypes to Agrobacterium-mediated delivery of Chickpea chlorotic dwarf virus (CpCDV) genome and identification of resistance source. Applied Microbiology and Biotechnology , 97 (21). pp. 9491-9501.

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Abstract

Chickpea stunt disease caused by Chickpea chlorotic dwarf virus (CpCDV) (genus Mastrevirus, family Geminiviridae) is the most important biotic stress affecting chickpea crops worldwide. A survey conducted on the incidence of stunt disease clearly revealed high incidence of the disease with severe symptom expression in both indigenous and imported genotypes. To manage the disease in a sustainable way, resistant genotypes need to be bred by adopting objective and precise assessment of the disease response of chickpea genotypes. At present, evaluation of CpCDV resistance is conducted on the basis of natural infection in the field, which is bound to be erroneous due to vagaries in vector population. To circumvent the above problems, we devised an agroinoculation technique that involves the delivery of viral genomic DNA through Agrobacterium tumefaciens. An objective scoring system assigning quantitative value to different symptoms has been evolved to assess the response of chickpea genotypes to CpCDV inoculation. Using the inoculation and scoring techniques, we screened 70 genotypes, which helped in differentiating field resistance that is more due to resistance to vector feeding than resistance to the virus

Item Type: Article
Uncontrolled Keywords: Chickpea, Mastrevirus , Stunt, Leafhopper
Author Affiliation: Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110012, India
Subjects: Plant Protection
Crop Improvement
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 09 Feb 2014 11:17
Last Modified: 09 Feb 2014 11:17
Official URL: http://dx.doi.org/10.1007/s00253-013-5162-9
URI: http://eprints.icrisat.ac.in/id/eprint/12448

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