Priyanka, S.R. and Uppalapati, S.R. and Kingston, J.J. and et al, . (2013) Development of ISSR-derived SCAR marker-targeted PCR for identification of Aspergillus section Flavi members. Letters in Applied Microbiology. pp. 1-9.
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Abstract
Aspergillus section Flavi is a heterogeneous fungal cluster including some of the most economically important Aspergillus species. The section is comprised of toxigenic and nontoxigenic aspergilli that are phenotypically undistinguishable. The aim of this study was to develop a genetic marker specific to Aspergillus section Flavi on the whole. Based on inter–simple sequence repeat (ISSR) fingerprinting profiles of major Aspergillus section Flavi members, a sequence-characterized amplified region (SCAR) marker was identified. Primers were designed in the conserved regions of the SCAR marker and were utilized in a PCR for concurrent identification of major members of the section. The detection level of the SCAR-PCR was found to be 0·1 ng purified DNA, and when applied to 45 naturally contaminated food samples, 28 samples were found infected with Aspergillus section Flavi members. The present SCAR-PCR is rapid and less cumbersome unlike conventional identification techniques....
| Item Type: | Article |
|---|---|
| Author Affiliation: | Defence Food Research Laboratory, Siddarthnagar, Mysore, Karnataka, India |
| Subjects: | Crop Improvement |
| Divisions: | General |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 21 Jan 2014 06:39 |
| Last Modified: | 21 Jan 2014 06:39 |
| Official URL: | http://dx.doi.org/10.1111/lam.12207 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/12405 |
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