Segregation of Fusarium wilt susceptibility linked DNA marker in susceptible resistance cross of chickpea.

Soregaon, C.D. and Satish, D. and Kumar, D. and Shinde, G. and Ravikumar, R.L. (2010) Segregation of Fusarium wilt susceptibility linked DNA marker in susceptible resistance cross of chickpea. Karnataka Journal of Agricultural Sciences , 23 (2). 320-321 .

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Abstract

The segregation of an allele-specific associated primer (ASAP) marker for resistance to Fusarium wilt (caused by Fusarium oxysporum f.sp. ciceri) that is linked to susceptibility to race 1 of the pathogen at the H1 locus was studied in the cross between the highly susceptible chickpea genotype JG 62 and the resistant genotype WR 315. The parents, F1 plants, and 119 F2 plants were analysed for the presence of the ASAP marker. The specific fragment linked to H1 was present in the susceptible parent JG 62 and the F1 generation, but was absent in the resistant genotype WR 315. The ASAP marker was linked to susceptibility locus H1 and susceptibility (H1) was dominant over resistance (h1). Among the 119 F2 segregants, fragment CS-27700 ASAP marker linked to H1 was present in 90 plants, but was absent in the remaining 29 plants. The goodness of fit for the 3:1 segregation ratio showed the single locus segregation of the linked marker, supporting the contention that the parents differ at the locus linked to the dominant marker.

Item Type: Article
Author Affiliation: Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad - 580 005, India.
Subjects: Crop Improvement > Biotechnology
Divisions: Chickpea
Depositing User: Mr T L Gautham
Date Deposited: 18 Oct 2010 16:06
Last Modified: 18 Oct 2010 16:06
URI: http://eprints.icrisat.ac.in/id/eprint/121

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