From genomics to functional markers in the era of next-generation sequencing

Salgotra, R.K. and Gupta, B.B. and Stewart Jr, C.N. (2013) From genomics to functional markers in the era of next-generation sequencing. Biotechnology Letters. pp. 1-10.

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Abstract

The availability of complete genome sequences, along with other genomic resources for Arabidopsis, rice, pigeon pea, soybean and other crops, has revolutionized our understanding of the genetic make-up of plants. Next-generation DNA sequencing (NGS) has facilitated single nucleotide polymorphism discovery in plants. Functionally-characterized sequences can be identified and functional markers (FMs) for important traits can be developed at an ever-increasing ease. FMs are derived from sequence polymorphisms found in allelic variants of a functional gene. Linkage disequilibrium-based association mapping and homologous recombinants have been developed for identification of “perfect” markers for their use in crop improvement practices. Compared with many other molecular markers, FMs derived from the functionally characterized sequence genes using NGS techniques and their use provide opportunities to develop high-yielding plant genotypes resistant to various stresses at a fast pace.

Item Type: Article
Additional Information: Crop plants, Functional markers, Genomic selection, Next generation DNA sequencing, Plant biotechnology, plant breeding, polymorphisms
Author Affiliation: Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, Chatha, Jammu, 190008, J & K, India
Subjects: Crop Improvement
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 25 Oct 2013 09:05
Last Modified: 25 Oct 2013 09:05
Official URL: http://dx.doi.org/10.1007/s10529-013-1377-1
URI: http://eprints.icrisat.ac.in/id/eprint/12039

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