Sankara, P. and Rao, P.V.S. and Strange, R.N. (1996) Differential accumulation of phytoalexins in leaves of susceptible and resistant genotypes of groundnut (Arachis hypogaea L.) inoculated with Puccinia arachidis Speg. Journal of Phytopathology , 144 (11-12). pp. 527-532.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
Restricted to ICRISAT researchers only |
Abstract
The accumulation of phytoalexins in a rust-susceptible (KH 241-D) and a rust-resistant (PI 259747) genotype of groundnut, 2, 6, 15 and 25 d after inoculation with P. arachidis was investigated. Within 2 d of inoculation of the resistant genotype, total phytoalexins reached 35 µg/g FW, remained close to this level for a further 13 d and reached 104 µg/g 25 d after inoculation. The susceptible genotype accumulated 4 µg/g FW 2 d after inoculation which increased gradually to 13, 23 and 52 µg/g FW at 6, 15 and 25 d, respectively. Extracts from uninoculated leaves of both genotypes showed the presence of an antifungal compound which appeared as a diffuse spot in a TLC bioassay. This was absent in inoculated leaves and did not correspond to any peak detected by HPLC. Compounds with UV spectra corresponding to isoflavanones were the major component of the phytoalexin response. The phytoalexins formononetin, daidzein and medicarpin comprised a minor component. A further compound with a retention time of 23.0 min on HPLC and a λ max of 280 nm only accumulated in inoculated leaves of the resistant genotype
| Item Type: | Article |
|---|---|
| Additional Information: | SNNigam collections |
| Author Affiliation: | Université de Ouagadougou, Ouagadougou, Burkina Faso, Africa |
| Subjects: | Plant Protection Statistics and Experimentation Crop Improvement |
| Divisions: | Groundnut |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 23 Sep 2013 05:02 |
| Last Modified: | 23 Sep 2013 05:02 |
| Official URL: | http://dx.doi.org/10.1111/j.1439-0434.1996.tb00294... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11763 |
Actions (login required)
 |
View Item |
