Divakara, S.T. and Santosh, P. and Aiyaz, M. and et al, . (2013) Molecular identification and characterization of Fusarium spp., associated with sorghum seeds. Journal of the Science of Food and Agriculture. pp. 1-21.
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Abstract
Fusarium spp., are not only pathogenic to plants but are also known as toxin producers that negatively affect animal and human health. The identification of Fusarium species still remains one of the most critical issues in fungal taxonomy. Different strains of Fusarium spp., were isolated from sorghum seed samples and identified at the molecular level by tef-1α gene amplification. Multiplex PCR (mPCR) assay was developed to differentiate toxigenic and non toxigenic Fusarium species by designing a primer for Fum21gene along with Fum1 and Fum8 genes. Competitive direct-enzyme linked immunosorbent assay (CD-ELISA) was employed to assess the fumonisin producing ability of Fusarium spp. Phylogenetic analyses were performed using partial sequences of the tef-1α and ISSR markers of different Fusarium spp.
| Item Type: | Article |
|---|---|
| Additional Information: | The authors are grateful to the Department of Biotechnology (DBT), Govt. of India, (Project- BT/PR9864/AGR/05/391/2007 Dated: 6-6-2008) for the financial support through major research project. |
| Uncontrolled Keywords: | Sorghum; Fusarium spp; Translation elongation factor 1-α gene; ISSR markers |
| Author Affiliation: | Department of Studies in Biotechnology, University of Mysore, Mysore-, Karnataka, India |
| Subjects: | Plant Production |
| Divisions: | Sorghum |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 13 Sep 2013 05:54 |
| Last Modified: | 13 Sep 2013 05:54 |
| Official URL: | http://dx.doi.org/10.1002/jsfa.6380 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11710 |
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