Li, L. and Zhu, F. and Liu, H. and et al, . (2013) Isolation and expression analysis of defense-related genes in sorghum–Colletotrichum sublineolum interaction. Physiological and Molecular Plant Pathology. pp. 1-8.
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Abstract
Anthracnose caused by the fungal pathogen Colletotrichum sublineolum is one of the most devastating diseases in sorghum. To better understand the molecular basis of sorghum–C. sublineolum interactions, a cDNA library enriched for defense response mRNAs was constructed by suppression subtractive hybridization (SSH) of inoculated mesocotyl tissues of a resistant cultivar (cv. DK18) vs. non-inoculated samples. A total of forty pathogen-inducible genes involved in cell rescue and defense, signal transduction, abiotic stress, secondary metabolism and protein degradation were identified, implicating a complex defense response to the anthracnose pathogen. Gene expression analyses demonstrated that all the genes were induced by C. sublineolum and two non-host pathogens. However, the expression patterns of nineteen genes suggested that they are regulated differently during host and non-host defense responses in sorghum. Genes encoding an ABA-responsive protein, a leucine-rich repeat protein, a flavonoid 3′-hydroxylase and a glutathione S-transferase were induced in the resistant cultivar but their expression was hardly detected in susceptible plants, suggesting that they play significant roles in anthracnose resistance. This study provides an efficient resource for sorghum defense-related gene discovery that may further enhance the investigation of molecular mechanisms of defense responses in sorghum toward fungal pathogens.
| Item Type: | Article |
|---|---|
| Additional Information: | This work was supported by the grants from the National Basic Research Program of China (2013CB127803), the National Natural Science Foundation of China (31201472 and 31171799), and the HKU Seed Funding Program for Basic Research (201011159033). |
| Uncontrolled Keywords: | Defense response; Fungal pathogen; Suppression subtractive hybridization (SSH); Sorghum; Colletotrichumsublineolum |
| Author Affiliation: | Institute of Agro-Products Processing Science & Technology, Chinese Academy of Agricultural Sciences, Beijing, China |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 13 Sep 2013 05:23 |
| Last Modified: | 13 Sep 2013 05:23 |
| Official URL: | http://dx.doi.org/10.1016/j.pmpp.2013.08.005 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11705 |
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