Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions

Bucher, E. and Sijen, T. and Haan, P. de and Goldbach, R. and Prins, M. (2003) Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. Journal of Virology Vol. No. 2 pp. 1329-1336, 77 (2). pp. 1329-1336.

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Abstract

Posttranscriptional silencing of a green fluorescent protein (GFP) transgene in Nicotiana benthamiana plants was suppressed when these plants were infected with tomato spotted wilt virus (TSWV), a plant-infecting member of the Bunyaviridae. Infection with TSWV resulted in complete reactivation of GFP expression, similar to the case for potato virus Y, but distinct from that for Cucumber mosaic virus, two viruses known to carry genes encoding silencing suppressor proteins. Agrobacterium-based leaf injections with individual TSWV genes identified the NSS gene to be responsible for the RNA silencing-suppressing activity displayed by this virus. The absence of short interfering RNAs in NSS-expressing leaf sectors suggests that the tospoviral NSS protein interferes with the intrinsic RNA silencing present in plants. Suppression of RNA silencing was also observed when the NS3 protein of the Rice hoja blanca tenuivirus, a nonenveloped negative-strand virus, was expressed. These results indicate that plant-infecting negative-strand RNA viruses carry a gene for a suppressor of RNA silencing.

Item Type:	Article
Author Affiliation:	Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, Netherlands
Subjects:	Crop Improvement > Genetics/Genomics Crop Improvement > Plant Breeding
Divisions:	Other Crops
Depositing User:	Sandhya Gir
Date Deposited:	18 Oct 2010 09:22
Last Modified:	21 Oct 2010 03:21
Official URL:	http://dx.doi.org/10.1128/JVI.77.2.1329-1336.2003
URI:	http://eprints.icrisat.ac.in/id/eprint/117

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