Analysis of a phenylalanine ammonia-lyase gene sequence from Arachis hypogaea L. and its transcript abundance in induced systemic resistance against Sclerotium rolfsii

Tonelli, M.L. and Ibanez, F. and Taurian, T. and et al, . (2013) Analysis of a phenylalanine ammonia-lyase gene sequence from Arachis hypogaea L. and its transcript abundance in induced systemic resistance against Sclerotium rolfsii. Journal of Plant Pathology , 95 (1). pp. 191-195.

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Abstract

Phenylalanine ammonia-lyase (PAL) is an important plant enzyme in pathogen defense. The purpose of this work was to analyze the phylogenetic relationships of a partial PAL gene sequence from Arachis hypogaea L. (peanut) cv. Tegua (designated AhPAL), a PAL sequence from cultivar JL24 and PAL sequences from other legumes and non-legumes plants. In addition, changes in the AhPAL transcript abundance related to induced systemic resistance (ISR) were also evaluated. AhPAL sequence shows high level of similarity with PAL genes and proteins from several plant species, including legumes and non-legumes. Quantification of relative transcript abundance indicated that AhPAL could be involved in the A. hypogaea cv. Tegua response towards the phytopathogen Sclerotium rolfsii.

Item Type: Article
Additional Information: This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Secretaría de Ciencia y Técnica de la Universidad Nacional de Río Cuarto (SECYT-UNRC), CONICET. M.L. T. has a postdoctoral fellowship from CONICET, F.I., A.F and T.T. are members of research career of CONICET, Argentina. Real Time-PCR assays were done in Dr. José M. Argüello’s lab in Worcester Polytechnic Institute, Worcester, MA, USA, under a Fulbright Fellowship given to M.L.T.
Uncontrolled Keywords: Biotic stress, Bacillus sp., defense response, peanut, phenylalanine ammonia lyase
Author Affiliation: Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-quimicas y Naturales, Universidad Nacional de Río Cuarto, Argentina.
Subjects: Statistics and Experimentation
Crop Improvement
Divisions: Groundnut
Depositing User: Mr Arbind Seth
Date Deposited: 29 Aug 2013 14:18
Last Modified: 29 Aug 2013 14:18
Official URL: http://dx.doi.org/10.4454/JPP.V95I1.029
URI: http://eprints.icrisat.ac.in/id/eprint/11574

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