Datta, B. and Chakraborty, P.K. (2013) Characterization of a Tn5-induced siderophore over-producing mutant of Rhizobium nodulating chickpea. Journal of Mycopathological Research , 51 (1). pp. 39-47.
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Abstract
Rhizobium BICC 651, a chickpea (Cicer arietinum L.) nodulating strain, was mutagenised with transposon Tn5. The resultant mutant colony bank was screened for mutants on chrome azurol sulfone (CAS) plates for over-production of siderophore and, thus, two mutants were isolated. These mutants had higher level of siderophore in the culture filtrate than their parent strain under iron-limiting condition and were repressed for siderophore production in presence of iron (50 µm FeCl3), as such were not considered as siderophore regulatory mutants. One of these mutants designated as B00I was found to be impaired in the uptake of 59Fe-siderophore complex within the cell. A BamHI genomic DNA fragment adjacent to &e posterior end of Tn5 in the mutant was amplified by inverse PCR following self-ligation and was sequenced. Upon BLASTX search, part of the sequence of the fragment (265 bp) adjacent to Tn5 was found to have its highest homologs (90%) to that of siderophore interacting protein of Agrobacterium tumefaciens CCNWGSO2S6 suggesting that Tn5 interrupted the gene that codes for a protein required for uptake of ferri-siderophore complex wifiin the cell. Symbiotic efficiency of the mutant was examined and was found to produce about 30% higher number of nodules per plant as compared to its parent strain
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chickpea nodulation, Rhizobin siderophore-over-producing mutant,Ferrisiderophore complex uptake |
| Author Affiliation: | Department of Botany, University of Kalyani, Kalyani, Nadia, West Bengal, Pin - 741 235, India |
| Subjects: | Crop Improvement Plant Physiology and Biochemistry > Plant Physiology |
| Divisions: | Chickpea |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 27 Aug 2013 06:42 |
| Last Modified: | 27 Aug 2013 06:42 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11566 |
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