Kadiri, A. and Ighilhariz , Z. and Bouabdallah , L. and Benbayer, Z. (2013) Explant source, genotype and plant growth regulators effects on chickpea (Cicer arietinum L.) callus induction. International Journal of Agriculture and Crop Sciences, 5 (10). pp. 1067-1073.
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Abstract
Callus induction of was studied in three leaflets and internodes chickpea cultivars, ICC 3996 C, Zouaoui and Flip 82 150 C on MS (Murashige and Skoog, 1962) nutrient medium, supplemented with several hormonal combinations of 2, 4-D (2,4-Dichlorophenoxyacetic acid), NAA (1-Naphthaleneacetic acid) and BAP (6-Benzylaminopurine). The results showed that rates of callus production was significantly influenced at 5% level, by individual and interactive, genotype, explants source, hormonal balance added to the nutrient medium effects. Among the genotypes tested, Zouaoui cultivar showed better callogenic ability (61, 66%) compared to ICC 3996 C (50, 66%) and Flip 82 150 C (45, 75%). The two types of explants explained different reactivity. Internodes were significantly more callogenic and formed 61, 66% of produced callus, whereas with leaflets, only 45% were obtained. In addition, from the range of hormonal regime tested, MS medium containing 1 mg/l BAP 0, 5 mg 2, 4-D, or 0,5 mg/l NAA with 2,25 mg/l BAP, expressed the greatest callus formation rate with respectively 76,80% and 71,92%. However, MS containing 2, 4-D or NAA without cytokinine, showed low percentages of produced callus
| Item Type: | Article |
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| Uncontrolled Keywords: | Cicer arietinum L., Genotype, Growth regulators, Iinternodes, Leaflets |
| Author Affiliation: | Biology Department, Sciences Faculty, University of Oran, Oran, Algeria |
| Subjects: | Plant Production Crop Improvement |
| Divisions: | Chickpea |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 23 Aug 2013 09:44 |
| Last Modified: | 23 Aug 2013 09:44 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11526 |
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