Genetic variation and relationships of Zea mays and Sorghum species using RAPD-PCR and SDS-PAGE of seed proteins

Osman, G. and Munshi, A. and Altf, F. and et al, . (2013) Genetic variation and relationships of Zea mays and Sorghum species using RAPD-PCR and SDS-PAGE of seed proteins. African Journal of Biotechnology, 12 (27). pp. 4269-4276.

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Abstract

Genetic relationship between some species of Zea mays and Sorghum was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of seed protein and random amplification of polymorphic DNA (RAPD-PCR) markers. According to SDS-PAGE analysis, 78 band were identified across the studied species. The number of bands varies from 17 bands in sample number 5 to 6 in sample number 6. Analysis of RAPD-PCR of DNA provided more precise information concerning relationships between Zea mays and Sorghum species than SDS-PAGE analysis. A remarkable result from this study was identifying a close relationship between Zea mays spp. mays and Zea mays spp. Mexicana. Further support comes from the molecular data of RAPD, which indicate that close relationship between Sorghum valgare and Sorghum bicolor

Item Type:	Article
Uncontrolled Keywords:	Zea mays, Sorgum volgare pres, Punciu milia ceaum L. protein, random amplification of polymorphic DNA
Author Affiliation:	Biology Department, Faculty of Science, Umm-Al-Qura University, Makka 673, Saudi Arabia
Subjects:	Crop Improvement
Divisions:	Sorghum
Depositing User:	Mr T L Gautham
Date Deposited:	27 Aug 2013 03:40
Last Modified:	27 Aug 2013 03:40
Official URL:	http://www.academicjournals.org/ajb/abstracts/abs2...
URI:	http://eprints.icrisat.ac.in/id/eprint/11522

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