Nodari , R.O. and Tsai , S.M. and Guzman , P. and et al, . (1993) Toward an integrated linkage map of common bean. III. Mapping genetic factors controlling host-bacteria interactions. Genetics, 134 (1). pp. 341-350.
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Abstract
Restriction fragment length polymorphism (RFLP)-based genetic linkage maps allow us to dissect the genetic control of quantitative traits (QT) by locating individual quantitative trait loci (QTLs) on the linkage map and determining their type of gene action and the magnitude of their contribution to the phenotype of the QT. We have performed such an analysis for two traits in common bean, involving interactions between the plant host and bacteria, namely Rhizobium nodule number (NN) and resistance to common bacterial blight (CBB) caused by Xanthomonas campestris pv. phaseoli. Analyses were conducted in the progeny of a cross between BAT93 (fewer nodules; moderately resistant to CBB) and Jalo EEP558 (more nodules; susceptible to CBB). An RFLP-based linkage map for common bean based on 152 markers had previously been derived in the F2 of this cross. Seventy F2-derived F3 families were inoculated in separate greenhouse experiments with Rhizobium tropici strain UMR1899 or X. c. pv. phaseoli isolate isolate W18. Regression and interval mapping analyses were used to identify genomic regions involved in the genetic control of these traits. These two methods identified the same genomic regions for each trait, with a few exceptions. For each trait, at least four putative QTLs were identified, which accounted for approximately 50% and 75% of the phenotypic variation in NN and CBB resistance, respectively. A chromosome region on linkage group D7 carried factor(s) influencing both traits. In all other cases, the putative QTLs affecting NN and CBB were located in different linkage groups or in the same linkage group, but far apart (more than 50 cM). Both BAT93 and Jalo EEP558 contributed alleles associated with higher NN, whereas CBB resistance was always associated with BAT93 alleles. Further investigations are needed to determine whether the QTLs for NN and CBB on linkage group D7 represent linked genes or the same gene with pleiotropic effects. Identification of the QTLs raises the possibility of initiating map-based cloning and marker-assisted selection for these traits.
| Item Type: | Article |
|---|---|
| Additional Information: | This research was funded in part by the Agency for International Development (Washington, D.C.) under the PSTC and Bean/Cow- pea CRSP programs, and Computing Services at University of California, Davis. R.O.N. holds a fellowship from CNPq, Brazil. S.M.T. holds fellowships from CNPq and FAPESP, Brazil. We thank the following individuals: P. GRAHAM for providing the Rhizobium strain; E. LANDER and S. LINCOLN for the current version of MAPMAKER-QTL; J. KIPE-NOLT for providing the Nod- mutant; and S. SINCH for advice on the choice of parents. |
| Uncontrolled Keywords: | Integrated linkage map, common bean. III., Mapping genetic Mapping, host-bacteria |
| Author Affiliation: | Department of Agronomy and Range Science, University of California, Davis |
| Subjects: | Soil Science and Microbiology > Microbiology Crop Improvement |
| Divisions: | Common Bean |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 26 Aug 2013 06:22 |
| Last Modified: | 26 Aug 2013 06:22 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11467 |
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