Determination of aflatoxins in grains and raw peanuts by a rapid procedure with fluorometric analysis

Malone, B.R. and Humphrey, C.W. and Romer, T.R. and Richard, J.L. (2000) Determination of aflatoxins in grains and raw peanuts by a rapid procedure with fluorometric analysis. Journal of AOAC International , 83 (1). pp. 95-98.

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Abstract

A rapid, quantitative, inexpensive and efficient method was developed to determine aflatoxins in corn [maize], corn meal, popcorn, rice, wheat, cottonseed and peanuts [groundnuts]. Samples were ground and extracted with methanol-water (80+20). A portion of the extract was cleaned up by passage through a solid-phase separatory column, 500 µl purified extract was derivatized with a bromine reagent, and fluorescence of the solution was immediately quantified with a calibrated fluorometer containing a broad wavelength pulsed xenon light source. This method quantified aflatoxin from 5 to 5000 p.p.b. without dilution and was linear when applied to samples of noncontaminated maize spiked at 0 to 5000 µg/g aflatoxin B1. Correlation coefficients of the method with LC for multiple analyses for maize (n=34), cottonseed (n=32), and groundnuts (n=11) were 0.999, 0.995 and 0.980, respectively. Individual analyses may be conducted in less than 5 min, and grouping of samples is unnecessary. The sensitivity of the method for maize was 5 p.p.b. and the fluorometer, under the operating conditions, has a limit of detection of 0.6 ng aflatoxin B1

Item Type:	Article
Additional Information:	SNNigam collections
Author Affiliation:	Romer Labs, Inc., 1301 Stylemaster Dr, Union, MO 63084-1156, USA.
Subjects:	Plant Protection Postharvest Management > Food Technology Statistics and Experimentation
Divisions:	Groundnut
Depositing User:	Mr T L Gautham
Date Deposited:	08 Aug 2013 11:20
Last Modified:	08 Aug 2013 11:20
URI:	http://eprints.icrisat.ac.in/id/eprint/11388

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