Fan, T.S.L. and Chu, F.S. (1984) Indirect enzyme-linked immunosorbent assay for detection of aflatoxin B1 in corn and peanut butter. Journal of Food Protection , 47 (4). pp. 263-266.
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Abstract
The method described involves coating aflatoxin B1 (AFB1) polylysine conjugate on microtitre plates as immobilized antigen, followed by incubation with free toxin standard or sample extract and anti-aflatoxin antibody from rabbits. The amount of antibody bound to the solid phase was determined by subsequent incubation with a secondary antibody conjugated with an enzyme (goat anti-rabbit IgG-horseradish peroxidase conjugate) and reaction with the chromogenic substrate. Aflatoxins were extracted from groundnut butter and maize meal and dissolved in assay buffer for ELISA. Using the technique 79.5-98.6% and 68-97% of AFB1 added in the range 5-40 µg/kg to the maize meal and groundnut butter were recovered, respectively. The specificity for AFB1 as that obtained from the direct ELISA, with an additional advantage that much less antibody was required for the assay
| Item Type: | Article |
|---|---|
| Additional Information: | SNNigam collections |
| Author Affiliation: | Food Res. Inst., Univ. Wisconsin, Madison, Wis. 53706, USA. |
| Subjects: | Plant Protection Soil Science and Microbiology > Microbiology |
| Divisions: | Groundnut |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 08 Aug 2013 10:35 |
| Last Modified: | 08 Aug 2013 10:35 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11372 |
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