Resistance gene analogues in sugarcane and sorghum and their association with quantitative trait loci for rust resistance

McIntyre, C.L. and Casu, R.E. and Drenth, J. and Knight, D. and Whan, V.A. and Croft, B.J. and Jordan, D.R. and Manners, J.M. (2005) Resistance gene analogues in sugarcane and sorghum and their association with quantitative trait loci for rust resistance. Genome, 48 (3). pp. 391-400.

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Abstract

Fifty-four different sugarcane resistance gene analogue (RGA) sequences were isolated, characterized, and used to identify molecular markers linked to major disease-resistance loci in sugarcane. Ten RGAs were identified from a sugarcane stem expressed sequence tag (EST) library; the remaining 44 were isolated from sugarcane stem, leaf, and root tissue using primers designed to conserved RGA motifs. The map location of 31 of the RGAs was determined in sugarcane and compared with the location of quantitative trait loci (QTL) for brown rust resistance. After 2 years of phenotyping, 3 RGAs were shown to generate markers that were significantly associated with resistance to this disease. To assist in the understanding of the complex genetic structure of sugarcane, 17 of the 31 RGAs were also mapped in sorghum. Comparative mapping between sugarcane and sorghum revealed syntenic localization of several RGA clusters. The 3 brown rust associated RGAs were shown to map to the same linkage group (LG) in sorghum with 2 mapping to one region and the third to a region previously shown to contain a major rust-resistance QTL in sorghum. These results illustrate the value of using RGAs for the identification of markers linked to disease resistance loci and the value of simultaneous mapping in sugarcane and sorghum.

Item Type: Article
Author Affiliation: CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Rd, St. Lucia, Brisbane QLD 4067, Australia
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Sorghum
Depositing User: Sandhya Gir
Date Deposited: 22 Dec 2010 20:47
Last Modified: 29 Dec 2010 19:34
Official URL: http://article.pubs.nrc-cnrc.gc.ca/RPAS/rpv?hm=HIn...
URI: http://eprints.icrisat.ac.in/id/eprint/1131

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