Zeng, X. and Small, D.P. and Wan, W. (2011) Statistical optimization of culture conditions for bacterial cellulose production by Acetobacter xylinum BPR 2001 from maple syrup. Carbohydrate Polymers, 85 (3). pp. 506-513.
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Abstract
Bacterial cellulose production by Acetobacter xylinum BPR 2001 was optimized using maple syrup as a carbon source. Twelve culture parameters were screened by the Plackett–Burman design and significant parameters were optimized by the response surface methodology using a three-level, four-factor Box–Behnken design. For fermentation in a rotary shaker, the optimal conditions for bacterial cellulose production were: maple syrup 30 g carbohydrate/l, (NH4)2SO4 3.3 g/l, KH2PO4 1 g/l, yeast extract 20 g/l, citric acid 1.6 g/l, trisodium citrate dehydrate 2.4 g/l, ethanol 0.5% (v/v), acetic acid 0.5 g/l, MgSO4·7H2O 0.8 g/l, inoculum age 3 days, inoculum volume 6% (v/v), shaking speed 135 rpm, and incubation temperature 25 °C. Comparison of bacterial cellulose production with maple syrup or pure sugars showed maple syrup was a suitable carbon source. This was the first demonstration of conversion of maple syrup, a plentiful renewable resource, into bacterial cellulose, a nanobiomaterial ideal for many applications.
Item Type: | Article |
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Additional Information: | This research was supported by the Natural Sciences and Engineering Research Council of Canada (to W.K.W.) and the National Natural Science Foundation of China (Grant No. 30900298) and Self-research Program of Wuhan University (Grant No. 3082013) (to X.Z.). One of us (X.Z.) |
Uncontrolled Keywords: | Acetobacter xylinum; Bacterial cellulose; Maple syrup; Optimization; Production; Biomaterials |
Author Affiliation: | College of Pharmacy, Wuhan University, Wuhan, Hubei 430072, PR China |
Subjects: | Postharvest Management > Food Technology |
Divisions: | General |
Depositing User: | Mr Siva Shankar |
Date Deposited: | 29 Jul 2013 08:28 |
Last Modified: | 29 Jul 2013 08:28 |
Official URL: | http://dx.doi.org/10.1016/j.carbpol.2011.02.034 |
URI: | http://eprints.icrisat.ac.in/id/eprint/11291 |
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