Statistical optimization of culture conditions for bacterial cellulose production by Acetobacter xylinum BPR 2001 from maple syrup

Zeng, X. and Small, D.P. and Wan, W. (2011) Statistical optimization of culture conditions for bacterial cellulose production by Acetobacter xylinum BPR 2001 from maple syrup. Carbohydrate Polymers, 85 (3). pp. 506-513.

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Abstract

Bacterial cellulose production by Acetobacter xylinum BPR 2001 was optimized using maple syrup as a carbon source. Twelve culture parameters were screened by the Plackett–Burman design and significant parameters were optimized by the response surface methodology using a three-level, four-factor Box–Behnken design. For fermentation in a rotary shaker, the optimal conditions for bacterial cellulose production were: maple syrup 30 g carbohydrate/l, (NH4)2SO4 3.3 g/l, KH2PO4 1 g/l, yeast extract 20 g/l, citric acid 1.6 g/l, trisodium citrate dehydrate 2.4 g/l, ethanol 0.5% (v/v), acetic acid 0.5 g/l, MgSO4·7H2O 0.8 g/l, inoculum age 3 days, inoculum volume 6% (v/v), shaking speed 135 rpm, and incubation temperature 25 °C. Comparison of bacterial cellulose production with maple syrup or pure sugars showed maple syrup was a suitable carbon source. This was the first demonstration of conversion of maple syrup, a plentiful renewable resource, into bacterial cellulose, a nanobiomaterial ideal for many applications.

Item Type: Article
Additional Information: This research was supported by the Natural Sciences and Engineering Research Council of Canada (to W.K.W.) and the National Natural Science Foundation of China (Grant No. 30900298) and Self-research Program of Wuhan University (Grant No. 3082013) (to X.Z.). One of us (X.Z.)
Uncontrolled Keywords: Acetobacter xylinum; Bacterial cellulose; Maple syrup; Optimization; Production; Biomaterials
Author Affiliation: College of Pharmacy, Wuhan University, Wuhan, Hubei 430072, PR China
Subjects: Postharvest Management > Food Technology
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 29 Jul 2013 08:28
Last Modified: 29 Jul 2013 08:28
Official URL: http://dx.doi.org/10.1016/j.carbpol.2011.02.034
URI: http://eprints.icrisat.ac.in/id/eprint/11291

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