Sathish, K.M. and Karrunakaran, C.M. and Anbuselvi, S. (2009) Production of lipase from Bacillus spp. using germinated maize oil and various carbon sources and effect of lipase activity on pH and temperature. International Journal of Biotechnology & Biochemistry, 5 (4). pp. 361-367.
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Abstract
Bacillus spp. was isolated from soil sample collected near Bharath University, Chennai, India. The lipase produced by the isolated Bacillus spp. was studied in this report. The Bacillus spp. grew optimally at pH 6.0 and temperature 50°C on nutrient agar. Effect of different carbon sources on lipase production was studied on maize oil, germinated maize oil, castor oil, coconut oil, olive oil, soybean oil and ground nut oil. The maximum production of lipase was found in olive oil and germinated maize oil at 45°C then its activity was found to be 30 U/ml and 26 U/ml. The production of lipase was done in 5 l Bioengineering fermentor. The oil from germinated maize was obtained through soxhlet apparatus using petroleum ether as a solvent and later the oil was separated from petroleum ether through fractional distillation then it is used for lipase production. The effect of pH and temperature on lipase was determined. This lipase produced was most active at pH 6.5 and at a temperature of 50°C
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Industrial Biotechnology, Bharath University, Selaiyur, Chennai, India |
| Subjects: | Plant Production |
| Divisions: | Maize |
| Depositing User: | Syamala |
| Date Deposited: | 22 Dec 2010 19:46 |
| Last Modified: | 29 Dec 2010 19:35 |
| Official URL: | http://www.indianjournals.com/ijor.aspx?target=ijo... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/1128 |
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